What temp should primers be stored at? They should be kept dry and not exposed to high tempera- tures (in excess of 150° F). If exposed to wet conditions or high temperatures, they may deteriorate, yielding mis- fires or poor ignition of the propellant powder. Primers do explode.
Can you thaw primers at room temp? Thaw at room temperature and then put primers on ice. Thawing on ice will damage primers more than at room temperature. Freeze/thaw cycles can damage primers.
How long are oligos stable at room temperature? Twist recommends storing Resuspended DNA for the following durations based on storage condition. Room Temperature for 3 months. 4° C for 12 months. -20° C for 24 months.
How long can primers stay in fridge? -20oC storage gives primers stability for about an year. You should not expect any significant degradation or quality loss in -80oC storage even after 2 years. The only concern should be the primers should be stored in multiple aliquots to prevent the whole stock to thaw & freeze repeatedly. Primers are cheap.