Does blood need to clot before you spin to get plasma? Plasma is produced when whole blood is collected in tubes that are treated with an anticoagulant. The blood does not clot in the plasma tube. The cells are removed by centrifugation. The supernatant, designated plasma is carefully removed from the cell pellet using a Pasteur pipette.
Does plasma allow blood to clot? Blood plasma contains fibrinogen, a large molecular weight (330 kDa), soluble protein. Conversion of fibrinogen into insoluble fibrin, and then cross-linking the fibrin, produces a tangled meshwork of filaments that comprises the blood clot. Plasma itself can clot, because it possesses fibrinogen.
How long does the blood need to clot before you centrifuge it? (Minimum clotting time is 30 minutes for patients with an intact clotting process.) After allowing the clot to form, insert the tube in the centrifuge, stopper end up. Operate the centrifuge for 10 minutes at the speed recommended by the manufacturer. Prolonged centrifugation may cause hemolysis.
How do you clot plasma? Hi Thomas and Vivek, your citrated or EDTA plasma can be clotted by addition of 1 part 250 mM CaCl2 to 10 parts of plasma. You need about 30 min at 37°C. Be sure that the tube you use is not too anticoagulant (polypropylene or irradiated polystyrene often inhibit thrombin generation).